The Two Steps Plus Detection System Peroxidase Goat Anti-Mouse/ Rabbit HRP + DAB, is a Ready-to-Use system that has been manufactured to give an optimal staining, when using the protocol advised in related IFU. Prior to staining, some routine Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections should be subjected to pre-treatment (HIER or digestive enzyme). The Two Steps Plus Detection System has Peroxidase that detects Mouse or Rabbit bound to an antigen in tissue sections and when a substrate like DAB is added, it produces a color change visible under a microscope. This polymer-complex is then visualized using a suitable substrate/chromogen also using this model instead of Two Steps Detection is more cost-effective. The antibodies are not provided but it is recommended to use the PathoSage antibodies.
Reference | Contents | Volume | PSW-DKI1003K-0150 |
|---|---|---|
Two Steps Plus Detection System Goat Anti-Mouse/ Rabbit HRP + DAB
| 150 Test
| |
1. Post-blocking (Ready-to-Use)
| 15 ml | |
2. Polymer Goat Anti-Mouse/Rabbit HRP (Ready-to-Use)
| 15 ml
| |
3. DAB Solution A: Buffered H202 (Ready-to-Use)
| 20 ml
| |
4. DAB Solution B: Concentrated DAB solution
| 1 ml | |
5. Peroxidase | 15 ml | |
PSW-DKI1003K-0550 | ||
Two Steps Plus Detection System Goat Anti-Mouse/ Rabbit HRP + DAB
| 550 Test
| |
1. Post-blocking (Ready-to-Use)
| 55 ml
| |
2. Polymer Goat Anti-Mouse/Rabbit HRP (Ready-to-Use)
| 55 ml | |
3. DAB Solution A: Buffered H202 (Ready-to-Use)
| 83 ml | |
4. DAB Solution B: Concentrated DAB solution
| 4 ml | |
5. Peroxidase | 55 ml | |
PSW-DKI1003K-1100 | Two Steps Plus Detection System Goat Anti-Mouse/ Rabbit HRP + DAB
| 1100 Test |
1. Post-blocking (Ready-to-Use)
| 110 ml | |
2. Polymer Goat Anti-Mouse/Rabbit HRP (Ready-to-Use)
| 110 ml | |
3. DAB Solution A: Buffered H202 (Ready-to-Use)
| 165 ml | |
4. DAB Solution B: Concentrated DAB solution
| 7.5 ml | |
5. Peroxidase | 110 ml |
